I don't know if this was the worst, but still...
Chief O'Hara: [about Egghead] He's a tough egg, Batman, the smartest man in the world.
Robin: The second smartest man in the world.
Chief O'Hara: Present company excepted, of course.
(I wonder who Chief was really referring to...)
The Best:
Batman: [Chief O'Hara grabs The Penguin with intent of arresting him] No, Chief, no.
Chief O'Hara: What?
Batman: He was merely baiting us. Don't swallow.
Robin: Have a heart, Chief, Penguin didn't mean any harm.
Commissioner Gordon: [almost illegible with a face full of custard pie] Didn't mean any harm?
Chief O'Hara: Hittin' a Police Commissioner in the puss with a pie?

